Calza L, Giuliani A, Fernandez M, Pirondi S, D’Intino G, Aloe L, Giardino L (2003) Neural stem cells and cholinergic neurons: Regulation by immunolesion and treatment with mitogens, retinoic acid, and nerve growth factor. Proc Natl Acad Sci U S A 100(12):7325-7330. doi: 10.1073/pnas.1132092100
Summary: The authors explore the influence of exogenous administration of hormones, cytokines, and neurotrophins on stem cells following a lesion. Rats were treated with 2 or 3 µg of 192-Saporin (Cat. #IT-01) into the cerebral ventricles, which induced a lesion of the cholinergic system in the basal forebrain. The surgery was followed by infusion of EGF, bFGF, and NGF into the lesioned area, as well as addition of retinoic acid to the food pellets. This pharmacological control of endogenous neural stem cells increased the number of proliferating cells in both lesioned and non-lesioned animals, as well as improved performance in a water maze test.
Related Products: 192-IgG-SAP (Cat. #IT-01)