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Immunohistochemical detection of prolactin-releasing peptide2 in the brain of the inshore hagfish Eptatretus burgeri.

Amano M, Amiya N, Yamamoto N, Osugi T, Tsutsui K (2019) Immunohistochemical detection of prolactin-releasing peptide2 in the brain of the inshore hagfish Eptatretus burgeri. Gen Comp Endocrinol 274:1-7. doi: 10.1016/j.ygcen.2018.12.005

Objective: To identify Prolactin-releasing peptide2 (PrRP2) in hagfish.

Summary: The study demonstrates, for the first time, that PrRP2 is expressed in the brain of inshore hagfish. The restricted distribution of PrRP2-ir fibers in the HYinf suggests that PrRP2 does not directly regulate the pituitary gland, but regulates the function of the HYinf where PQRFa and CRH are expressed.

Usage: Immunohistochemistry (1:1000). The authors clarified the cross-reactivity of the antibody against other CRH family peptides, such as urocortin-I, II, III, urotensin-I, and sauvagine: the cross-reactivity of anti-CRH against CRH family peptides was found to be less than 0.01%, indicating the specificity of the antibody.

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