Inaba H, Nonaka I, Hashimoto D, Hirono M, Morita S, Kimura H, Iwakura H, Akamizu T, Nakata M (2025) Immune tolerance induction using the thyrotropin receptor epitope 78-94 (p37) prevents Graves’ disease in HLA-DR3 transgenic mice. Front Immunol 16:1633350. doi: 10.3389/fimmu.2025.1633350 PMID: 41256857
Objective: To investigate whether pre-immunization with the immunodominant TSH-R–derived peptide spanning residues 78–94 (ISRIYVSIDVTLQQLES; p37) could induceimmune tolerance and prevent Graves’ disease in DR3 transgenic mice.
Summary: GD was induced by intramuscular injection of adenovirus encoding human TSH-R (Ad-TSH-R289). Mice were pretreated with p37 either as a single 50 mg dose or by step-up escalation protocol (0.05 mg, 0.5 mg, and 5 mg), with or without a final 50 mg dose. The authors findings demonstrate that step-up immunization with p37 induces antigen-specific immune tolerance and effectively prevents the development of GD in HLA-DR3 transgenic mice.
Usage: Expression of TSH-R (1-289) was confirmed by Western blot (AB-N16)
Related Products: TSH Receptor (A10) Mouse Monoclonal (Cat. #AB-N16)