Yao Z, Shi Z, Hu P, Wang C, Wang H (2026) Visualization of endosomal escape of intracellularly delivered protein with unexpected photochemical internalization of trypan blue. Adv Sci (Weinh) e16456. doi: 10.1002/advs.202516456 PMID: 41961480
Objective: To demonstrate trypan blue as a simple yet powerful tool for advancing intracellular protein delivery and tracking.
Summary: Upon 590 nm light irradiation, trypan blue (TB) acts as a photosensitizer, triggering photochemical internalization (PCI) that promotes endosomal escape and protein release into the cytosol, accompanied by recovery of GFP fluorescence. This TB-mediated PCI not only enhances delivery efficiency but also allows real-time visualization of protein binding and release.
Usage: 143B cells were treated with Saporin, M2/Saporin, and M2/TB/Saporin at different Saporin concentrations, with or without light irradiation.
Related Products: Saporin (Cat. #PR-01)