Ren L, Zhao Z, Chao Y, Yu P, Mei Z, Du B, Cheng Y (2025) Optimization of lipid nanoparticles with robust efficiency for the delivery of protein therapeutics to augment cancer immunotherapy. Adv Sci (Weinh) e2500844. doi: 10.1002/advs.202500844 PMID: 40056044
Objective: To develop a family of Lipid Nanoparticles (LNPs) with robust high efficiency in addressing the multiple barriers in cytosolic protein delivery by incorporating clinically approved ionizable lipids into traditional cationic LNPs. The authors investigated the in vivo delivery efficacy of the LNPs using saporin as the model protein.
Summary: The combination of cationic and ionizable lipids enables efficient protein binding and endosomal escape. Optimized LNPs efficiently deliver various proteins, including antibodies, enzymes, toxins, and Cas9 into living cells with reserved functions. The optimized LNPs successfully deliver therapeutic proteins such as saporin and interleukin-10 (IL-10) to inhibit tumor growth in several animal models.
Usage: Cell Viability and Apoptosis Assay: For evaluating in vitro saporin delivery efficacy, 143B cells were incubated with free saporin or saporin/LNP at saporin concentrations ranging from 0 to 61 nm. In Vivo Saporin Delivery by LNP: mice were intravenously injected with 100μL saporin or saporin/LNP once every two days. The doses of saporin and LNP in in vivo experiments were fixed at 50μg kg−1 and 2.4 mg lipid/kg, respectively.
Related Products: Saporin (Cat. #PR-01)