Yamaguchi S, Yamamoto K, Yamamoto R, Takamori S, Ishiwatari A, Minamihata K, Nagamune T, Okamoto A (2022) Intracellular protein photoactivation using sterically bulky caging. Chembiochem 23(22):e202200476. doi: 10.1002/cbic.202200476 PMID: 36173993
Objective: To develop an intracellular protein photoactivation method using sterically bulky caging.
Summary: Saporin was biotinylated and conjugated to streptavidin to block the active site of saporin. This temporarily inactivated protein was then activated via the cleaving of the streptavidin linker through light. This simple and versatile photoactivation method is a promising tool for studying spatio-temporal cellular events.
Usage: A sterically bulky caged Sap (cSap) was prepared via the two-step caging method using a biotinylated caging reagent (BCR). In the first step, Saporin (PR-01) was randomly modified with BCR via the amide coupling reaction. In the second step, streptavidin was conjugated with the biotin moiety on the BCR-modified Sap.
Related Products: Saporin (Cat. #PR-01)