Du R, Zhang Y, Wu S, Zixing W, Jing W (2025) Progress, opportunity, and perspective on long term preservation of extracellular vesicles. SSRN ssrn.5553381. doi: 10.2139/ssrn.5553381
Objective: To provide an overview of the biology, function, and biomedical application of Extracellular vesicles (EVs) and introduce the method to evaluate the storage stability of EVs.
Summary: While there are multiple methods available for EVs preservation, each approach comes with its advantage and limitation. At present, the optimal storage method for various components in EVs is still unknown. The incorporation of cryoprotectants has proven beneficial in enhancing EV preservation. However, it’s important to acknowledge that the concentration of cryoprotectants significantly influences the cryopreservation outcome. Current assessment to evaluate EVs preservation emphasize the alterations in the morphology, size, particle number, and protein of EVs during preservation, however, the description on EVs preservation efficiency has not be standardized.
Usage: When HeLa cell-derived EVs underwent lyophilization, the structure and particle size remained unchanged, the zeta-potential remained around -10 mV before and after lyophilization. However, when the EVs were engineered with arginine-rich cell-penetrating peptide (R16 peptide) and encapsulated with saporin (SAP), the biological activity of EVs were highly affected after lyophilization (Noguchi et al., 2019).
Related Products: Saporin (Cat. #PR-01)
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