Li Y, Liu J, Yao L, Mao T, Wang X, He Z, Lyu J, Wang W (2025) Synergistic effect of guanidinium and tertiary amine groups on boosting gene delivery. JACS Au 5(8):3951-3959. doi: 10.1021/jacsau.5c00592 PMID: 40881396
Objective: To design and develop a new versatile branched poly (β-aminoester) (PAE)-based delivery system by incorporating the synergistic effects of guanidinium and tertiary amine functional groups, resulting in enhanced nucleic acid (DNA/mRNA) delivery.
Summary: The results show that self-assembly occurs when polymers containing different functional groups are mixed, which changes the interactions between molecules and significantly affects the structure of the polyplex. This synergy was found to significantly enhance cellular uptake, contributing to improved transfection efficiency.
Usage: The protein delivery capabilities of these polymers were further evaluated by delivering the toxic protein Saporin (SAP) in HEK cells. The results showed that all polymers were effective, with cell killing rates exceeding 70%; however, no significant differences were observed among the polymers.
Related Products: Saporin (Cat. #PR-01)