Orciani C, Foret MK, Cuello AC, Carmo SD (2025) Long-term nucleus basalis cholinergic lesions alter the structure of cortical vasculature, astrocytic density and microglial activity in Wistar rats. Neurobiology of Aging 150:132-145. doi: 10.1016/j.neurobiolaging.2025.03.006 PMID: 40121723
Objective: To investigate the effects of the Basal forebrain cholinergic neurons (BFCNs) input on neurovascular unit (NVU) components.
Summary: To address this issue, the authors immunolesioned the nucleus basalis by administering injections of the cholinergic immunotoxin 192-IgG-SAP. Authors observed a significant reduction in cortical vesicular acetylcholine transporter-immunoreactive synapses. This was accompanied by changes in the diameter of cortical capillaries and precapillary arterioles, as well as lower levels of vascular endothelial growth factor A (VEGF-A). Additionally, the cholinergic immunolesion increased the density of cortical astrocytes and microglia in the cortex. The loss of nucleus basalis cholinergic input negatively impacts cortical blood vessels, NVU components, and microglia phenotype.
Usage: 192-IgG-SAP (2.6 mg/ml, IT-01) was injected at 0.5 μg/μl (1.0 μl/ hemisphere).
Related Products: 192-IgG-SAP (Cat. #IT-01)