Sun Y, Wu X, Li J, Radiom M, Mezzenga R, Verma CS, Yu J, Miserez A (2024) Phase-separating peptide coacervates with programmable material properties for universal intracellular delivery of macromolecules. Nat Commun 15(1):10094. doi: 10.1038/s41467-024-54463-z PMID: 39572548
Objective: To systematically manipulate the sequence of Phase-separating peptides (PSPs) to unravel the relationships between their molecular structure, the physical properties of the resulting coacervate microdroplets (CMs), and their delivery efficacy.
Summary: A few amino acid alterations are sufficient to modulate the viscoelastic properties of CMs towards either a gel-like or a liquid-like state as well as their binding interaction with cellular membranes, collectively enabling to tune the kinetics of intracellular cargo release. The authors also demonstrated that the optimized PSPs CMs display excellent transfection efficiency in hard-to-transfect cells such as primary fibroblasts and immune cells.
Usage: The cytotoxicity of the saporin-loaded (at various concentrations) or pristine CMs were evaluated using the Cell Counting Kit-8 (CCK-8).
Related Products: Saporin (Cat. #PR-01)