Balukova A, Bokea K, Barber PR, Ameer-Beg SM, MacRobert AJ, Yaghini E (2024) Cellular imaging and time-domain FLIM studies of meso-tetraphenylporphine disulfonate as a photosensitising agent in 2D and 3D models. Int J Mol Sci 25(8):4222. doi: 10.3390/ijms25084222 PMID: 38673807
Objective: To investigate the uptake and subcellular localization of TPPS2a and evaluate the photooxidative mechanism using reactive oxygen species (ROS) and lipid peroxidation probes combined with appropriate ROS scavengers.
Summary: Overall, it can be concluded that the photophysical properties of TPPS2a in cells are not markedly perturbed and that the fluorescence lifetime behavior is consistent with that observed in model systems where both porphyrin monomers and aggregates are present.
Usage: Authors previously studied the PDT and PCI efficacy of TPPS2a in HEY cells in 2D and 3D spheroid models using saporin as the chemotherapeutic agent.
Related Products: Saporin (Cat. #PR-01)
See Also:
- Hadi LM, Yaghini E, Stamati K, Loizidou M, MacRobert AJ. (2018) Therapeutic enhancement of a cytotoxic agent using photochemical internalisation in 3D compressed collagen constructs of ovarian cancer. Acta Biomater 81:80-92. doi: 10.1016/j.actbio.2018.09.041 PMID: 30267880
- Mohammad Hadi L, Stamati K, Yaghini E, MacRobert AJ, Loizidou M (2023) Treatment of 3D in vitro tumoroids of ovarian cancer using photochemical internalisation as a drug delivery method. Biomedicines 11(2):572. doi: 10.3390/biomedicines11020572 PMID: 36831108