Aikins AO, Little JT, Rybalchenko N, Cunningham JT (2022) Norepinephrine innervation of the supraoptic nucleus contributes to increased copeptin and dilutional hyponatremia in male rats. Am J Physiol Regul Integr Comp Physiol 323(5):R797-R809. doi: 10.1152/ajpregu.00086.2022 PMID: 36189988
Objective: Authors hypothesized that the A1/A2 noradrenergic neurons support chronic release of arginine vasopressin in cirrhosis leading to dilutional hyponatremia.
Summary: Dilutional hyponatremia associated with liver cirrhosis is due to inappropriate release of arginine vasopressin (AVP). The A1/A2 neurons stimulate the release of AVP from the supraoptic nucleus (SON) in response to nonosmotic stimuli. Injection of Anti-DBH-SAP (Cat. # IT-03) resulted in: 1) significantly reduced number of DBH immunoreactive A1/A2 neurons, 2) significantly reduced number of A1/A2 neurons immunoreactive to both DBH and Delta FosB positive neurons, 3) reduced number of SON neurons immunoreactive to both AVP and Delta FosB, 4) preventing the increase in plasma copeptin observed in vehicle-injected common bileduct (BDL) rats, and 5) normalizing plasma osmolality and hematocrit as compared with vehicle injection. The data suggest that A1/A2 neurons contribute to increased plasma copeptin and hypoosmolality in male BDL rats.
Usage: Bilateral injection of Anti-DBH-SAP into the supraoptic nucleus at (50 ng/500 nL)
Related Products: Anti-DBH-SAP (Cat. #IT-03)