Polito L (2024) Women’s special issue series: Biomedicines. Biomedicines 12(3):471. doi: 10.3390/biomedicines12030471 PMID: 38540085
Summary: Saporin is a single-chain ribosome-inactivating protein (RIP) with low toxicity in cells and animals. When the protein is linked to a carrier that facilitates cellular uptake, the protein can become highly and selectively toxic to the cellular target of the carrier. For this reason, saporin is often used to construct immunotoxins or other hybrid conjugates. The article by Bortolotti et al. examined the effect of the most frequently used heterobifunctional reagents on the saporin molecule, intending to insert a chemical bridge between the toxin and the carrier. The authors evaluated the capability of derivatized saporin to maintain its enzymatic properties, i.e., protein synthesis inhibition, deadenylation of DNA, and its biologic properties, i.e., in vitro cytotoxicity. Therefore, this research can be of interest for constructing saporin-based immuno conjugates when small molecules are considered carriers.
Related Products: Saporin (Cat. #PR-01)
See Also:
- Bolognesi, A.; Bortolotti, M.; Maiello, S.; Battelli, M.G.; Polito, L. Ribosome-Inactivating Proteins from Plants: A Historical Overview. Molecules 2016,21, 1627.
- Polito, L.; Djemil, A.; Bortolotti, M. Plant Toxin-Based Immunotoxins for Cancer Therapy: A Short Overview. Biomedicines 2016,4,12.
- Bortolotti, M.; Biscotti, F.; Zanello, A.; Bolognesi, A.; Polito, L. New Insights on Saporin Resistance to Chemical Derivatization with Heterobifunctional Reagents. Biomedicines 2023,11, 1214.