Trickle K, Dornan W (1996) The “combined” 192 IGG saporin lesion approach as an anima model of alzheimer’s disease. Illinois Wesleyan Univ Thesis.
Objective: Provide evidence that injections of 192-IgG-SAP both in the medial septal area and the nucleus basalis magnocellularis creates a reliable animal model of Alzheimer’s disease (AD).
Summary: 192-IgG-SAP (IT-01) is a selective cholinergic neurotoxin. Intraventricular injections of 192-IgG-SAP into the lateral ventricles induced a 80-90% reduction of acetylcholine levels in the cortex and hippocampus. Although studies have reported an impairment of spatial learning following treatment by 192-IgG-SAP, the effects observed may be due to loss of cerebellar Purkinje cells following intraventricular injection (i.c.v.). Authors propose that through a combined medial septum and nucleus basalis magnocellularis lesioning technique, a model for AD can be created in a rat that better reflects only damage to strictly cholinergic cells in the impacted regions of the brain.
Usage: Animals received three injections of 192-IgG-SAP into the medial septal area and two (bilateral) injections into the nucleus basalis magnocellularis.
Related Products: 192-IgG-SAP (Cat. #IT-01)