Wang J, Zhang S, Li Y, Xu Q, Kritzer JA (2024) Investigating the cytosolic delivery of proteins by lipid nanoparticles using the chloroalkane penetration assay. Biochemistry doi: 10.1021/acs.biochem.3c00614 PMID: 38334719
Objective: To investigate bovine serum albumin (BSA) protein encapsulation and release within polylysine/polyglutamate (PLys/PGlu) coacervates.
Summary: The findings emphasize the importance of ingredient addition sequence in coacervate formation and encapsulation rates, attributed to preference contact between oppositely charged proteins and poly(amino acid)s.
Usage: The positively-charged saporin and lysozyme protein exhibited the highest encapsulation efficiency when first combined with PGlu, followed by the addition of PLys in simulations of the coacervate encapsulation of saporin.
Related Products: Saporin (Cat. #PR-01)