Chan A (2024) Engineering small protein based inhibitors and biodegraders for cytosolic delivery and targeting of the undruggable proteome. Univ Pennsylvania Thesis.
Objective: Studying the delivery of saporin across the cell membrane encapsulated in lipid nanoparticles.
Summary: Protein payloads, like saporin and others, are given negatively charged regions which create binding sites for cationic lipids to encapsulate the protein. The lipid nanoparticles with saporin or other proteins inside have the potential to interact with many more targets within the cell because they now cross the cell barrier more efficiently.
Usage: Encapsulated Saporin [PR-01] in lipid nanoparticles using anionic polypeptide linkers and using it in vivo (Sun, 2022) and in vitro (Rui, 2019).
Related Products: Saporin (Cat. #PR-01)
See Also:
- Rui, Y. et al. Carboxylated branched poly(β-amino ester) nanoparticles enable robustcytosolic protein delivery and CRISPR-Cas9 gene editing. Sci Adv 5, (2019).
- Sun, Y. et al. Phase-separating peptides for direct cytosolic delivery and redox-activatedrelease of macromolecular therapeutics. Nat Chem 14, 274–283 (2022).