Salvioni L, Testa F, Barbieri L, Giustra M, Bertolini JA, Tomaino G, Tortora P, Prosperi D, Colombo M (2022) Saporin toxin delivered by engineered colloidal nanoparticles is strongly effective against cancer cells. Pharmaceutics 14(7):1517. doi: 10.3390/pharmaceutics14071517 PMID: 35890411
Objective: Using Saporin-containing iron oxide nanoparticles to selectively eliminate cancer cells.
Summary: Saporin [PR-01] is trapped inside of nanoparticle using iron oxide and polymers that opens and delivers payload dependent on its pH enviroment. The nanoparticle-SAP was then treated on healthy and cancerous cells and tested for its selectivity in killing cancerous cells and its internalization efficiency.
Usage: An iron oxide nanoparticle is coated in an amphiphilic polymer, then linker groups are attached to the polymer coating which then bind saporin molecules to the nanoparticle. In the cytotoxicity assays, SK-Br-3 cells are treated with this nanoparticle at concentrations of 25, 37.5, and 50 μg/mL. In internalization assays, SK-Br-3 cells are treated with fluorescently-labeled saporin-nanoparticles and assessed on fluorescence intensity.
Related Products: Saporin (Cat. #PR-01)