Rust A, Hassan HH, Sedelnikova S, Niranjan D, Hautbergue G, Abbas SA, Partridge L, Rice D, Binz T, Davletov B (2015) Two complementary approaches for intracellular delivery of exogenous enzymes. Sci Rep 5:12444. doi: 10.1038/srep12444 PMID: 26207613
Summary: Botulinum-derived proteases can be easily delivered into neuroendocrine cells using certain DNA lipofection reagents, revealing a novel action of botulinum enzyme type C in neuroendocrine tumor cells. Other uses were studied that could be applied to other bio-medically relevant proteins such as translation-inhibiting enzymes. Different cancer cell lines show that lipofectamine 3000 efficiently delivers enzymes into the cytosol. A macrophage cell line, J774.2, exhibited high sensitivity to extracellular proteins even without lipofection, enabling future high-throughput screening of bioactive enzymes.