Zhou Y, Zhang K, Wang F, Chen J, Chen S, Wu M, Lai M, Zhang Y, Zhou W (2023) Polypyrimidine tract binding protein knockdown reverses depression-like behaviors and cognition impairment in mice with lesioned cholinergic neurons. Front Aging Neurosci 15:1174341. doi: 10.3389/fnagi.2023.1174341 PMID: 37181622
Objective: Examine the mechanisms of how knockdown of the RNA-binding protein polypyrimidine tract binding protein (PTB) reverses depression-like behavior and cognition impairment in mice with lesioned cholinergic neurons.
Summary: A specific loss of cholinergic neurons in the horizontal limb of the diagonal band of broca (HDB) is correlated with depression and dysfunction of cognition in mice. The authors induced cholinergic neuron loss via injection of 192-IgG-SAP. This was followed by injection of either antisense oligonucleotides or adeno-associated virus-shRNA in the injured area of HDB to knockdown PTB. Knockdown of PTB by these two approaches was found to relieve depression-like behaviors and alleviate cognitive impairment and the findings suggest that supplementing cholinergic neurons after PTB knockdown may be a therapeutic approach to reverse depression-like behaviors and cognitive impairment.
Usage: 192 IgG-saporin (Cat. IT-01) was injected bilaterally into the HDB at a volume of 0.25 μl with a concentration of 1 μg/μL, per side.
Related Products: 192-IgG-SAP (Cat. #IT-01)