Yang Q, Liu N, Zhao Z, Liu X, Yin L (2023) Dynamically crosslinked nanocapsules for the efficient and serum-resistant cytosolic protein delivery. Nano Research doi: 10.1007/s12274-023-5978-2
Objective: Improve protein delivery with the synthesis of epigallocatechin gallate/low-molecular-weight polyethylenimine/2-acetylphenylboric acid (EPP)-protein nano-capsules.
Summary: A protein delivery strategy was created through the crosslinking of various markers on the protein surface, hence forming the EPP-protein nano-capsules. The EPP-protein nano-capsule allowed for acid-triggered dissociation of EPP-protein nano-capsules in the endolysosomes, which triggered efficient intracellular release of the native proteins. Acid dissociation showed high efficiency and universality for diversities of proteins with different molecular weights and isoelectric points, including enzymes, toxins, antibodies, and CRISPR-Cas9 ribonucleoprotein.
Usage: Delivered into in vivo 4T1 tumors in mice (PBS, saporin, or EPP-saporin nano-capsules were i.v. injected (0.5 mg saporin/kg) on day 0, 2, 4, and 6) and in vitro HeLa cells (seeded with 0, 0.05, 0.1, 0.2, 0.5, 1, and 2 μg/mL concentrations of Saporin or EPP-Saporin).
Related Products: Saporin (Cat. #PR-01)