Gong S, Qiu J, Thayumanavan S (2023) Self-assembly of epitope-tagged proteins and antibodies for delivering biologics to antigen presenting cells. J Am Chem Soc doi: 10.1021/jacs.3c09334 PMID: 38147631
Objective: To describe a simple self-assembly strategy for generating artificial immune complexes.
Summary: The built-in recognition domains in the antibody, viz. the Fab and Fc domains, are judiciously leveraged for cargo conjugation to generate the nanoassembly and macrophage targeting, respectively. A responsive linker is engineered into the nanoassembly for releasing the protein cargo inside the macrophages while ensuring stability during delivery.
Usage: Cytotoxicity assay to measure cell death with targeted saporin.
Related Products: Saporin (Cat. #PR-01)