Ebadi R, Kordi-Tamandani DM, Ghaedi K, Nasr-Esfahani MH (2018) Comparison of two different media for maturation rate of neural progenitor cells to neuronal and glial cells emphasizing on expression of neurotrophins and their respective receptors. 45(6):2377-2391. doi: 10.1007/s11033-018-4404-4 PMID: 30306506
Objective: To compare neural differentiation potential of two different media, NB + 5%ES-FBS + N2B27 and Ko-DMEM + 5%ES-FBS for conversion of mESC derived neural progenitors (NPs) into mature neural cells with emphasis on effect of these two media on neurotrophins and their respective receptors expression.
Summary: Results indicated that NB + 5%ES-FBS + N2B27 medium promoted neural differentiation process of mESCs and caused enhancement of neurotrophins protein expression in addition to their cognate receptors.
Usage: Western Blot (1:4000)
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