Ma R, Latif R, Davies TF (2015) Human embryonic stem cells form functional thyroid follicles. Thyroid doi: 10.1089/thy.2014.0537 PMID: 25585054
Summary: Lysates were prepared from single- and double-transfected cells by treating with 1 ·RIPA buffer for 20 min on ice. The samples were then centrifuged and treated with 5 · sample buffer at 100°C for 5 min and loaded on a 4–15% SDS-PAGE gel. Western blotting was performed according to a standard protocol.
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