Anacker A, Esser KH, Lenarz T, Paasche G (2022) Purification of fibroblasts from the spiral ganglion. Front Neurol 13:877342. doi: 10.3389/fneur.2022.877342 PMID: 35493807
Objective: To provide a purified preparation of cochlear fibroblasts, which can be used in vitro for further investigations.
Summary: Subcultivation of the primary spiral ganglion cells culture removed the neurons from the culture and increased the fibroblast to glial cell ratio in the preparations. Direct immunolabeling for the Thy1-glycoprotein and the p75NGFR-enabled fluorescence-based cell sorting. This resulted in a cell culture of cochlear fibroblasts with a purity of more than 99%.
Usage: Cell Sorting (1:400)
Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)