Joshi BS, Ortiz D, Zuhorn IS (2021) Converting extracellular vesicles into nanomedicine: loading and unloading of cargo. Materials Today Nano 16:100148. doi: 10.1016/j.mtnano.2021.100148
Objective: To provide a comprehensive overview of (i) methods for the loading of EVs with therapeutic cargo, (ii) methods for EV surface functionalization to direct EVs to target cells, and (iii) methods to stimulate cargo release from EVs.
Summary: Development of methodologies to offload the natural cargo of EVs and substitute it for a cargo of interest, i.e., similar to the generation of empty viral capsids, may further aid in higher loading efficiency and enhanced therapeutic effects with improved safety.
Usage: Saporin, a small (30 kDa) ribosome-inactivating protein that induces cytotoxicity by inhibiting protein synthesis,was successfully loaded into EVs by electroporation.
Related Products: Saporin (Cat. #PR-01)
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