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Behavioral effects of immunotoxin 192IgG-saporin depends on the type of its administration to rats.

Dobryakova Y, Bolshakov A, Zaichenko M, Stepanichev M, Markevich V (2018) Behavioral effects of immunotoxin 192IgG-saporin depends on the type of its administration to rats. FENS 2018 Abstracts F055. Federation of European Neuroscience Societies, Berlin, Germany.

Summary: It is known that degeneration of cholinergic neurons is one of key events during development of Alzheimer’s disease. We used immunotoxin 192IgG-saporin, a conjugate of antibody to p75/NFGR receptor with saporin, to induce the cholinergic deficit in the hippocampus. Here, we compared effects of intracerebroventricular (i.c.v.) and intraseptal injection of 192IgG-saporin on the learning performance in rats. Immunohistochemical analysis of the ChAT stained sections showed that both types of 192IgG-saporin injection led to a strong loss ChAT-positive neurons in septal area compared to control. Behavioral testing began 3 weeks after the injection. We found that, in Morris Water Maze, i.c.v. injected rats had longer latencies to reach the platform and higher distance swam compared to control when the animals learned to find platform. We found that during probe trial, when the platform was removed from the maze, i.c.v.-treated rats spent significantly less time in a quadrant, where the platform was during training, and swam shorter distance in it, as compared to the control animals. Rats treated intraseptally with the immunotoxin had no behavioral deficits in the Morris Water Maze. In the beam walking test both groups of rats showed small but significant reduction of motor performance (p<0.05). In contrast, locomotor and exploratory activity in the open field task was affected only by intraseptal toxin administration as compared to the control. In conclusion, our data suggest that different types of immunotoxin administration leads to different disturbances in behavior. The work was supported by Grant of Russian Science Foundation No 16-15-10403.

Related Products: 192-IgG-SAP (Cat. #IT-01)

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