Yan J, Price DL, Koliatsos VE (2001) Nestin expression in neurons of the medial septum/diagonal band in the adult rat. Neuroscience 2001 Abstracts 25.9. Society for Neuroscience, San Diego, CA.
Summary: Nestin is a marker for neuronal precursor cells in normal animals. In adult animals, nestin (+) cells are limited to the ventricular wall, hippocampus and the rostral migratory stream, where neurogenesis is known to persist throughout life. We are now reporting the existence of nestin (+) cells in the medial septum/diagonal band area based on immunocytochemical staining with different nestin antibodies. Many of these cells colocalize ChAT and nestin. In addition, some nestin (+) cells can be traced with the carbocyanin dye SP-DiI injected into the lateral ventricle to label cell lineages originating in the ependymal layer. Medial septal/diagonal band lesions by complete fimbria-fornix transections or 192-IgG-saporin conjugate injections into the ventricle cause an increase in BrdU (+) and nestin (+) cells in medial septum/diagonal band especially in anterior planes. We are currently double labeling the sections with BrdU and nestin or TUJ1. Our working hypothesis is that there may be ongoing neurogenesis in the medial septum/diagonal band in the adult brain, especially after injury or under pathological conditions and this may have implications for pathogenesis and treatment of Alzheimer’s disease.
Related Products: 192-IgG-SAP (Cat. #IT-01)