Schaevitz LR, Baxter MG, Stearns NA, Huang YY, Lappi DA, Berger-Sweeney J (2002) The efficacy of intraparenchymal anti-p75 immunotoxin on medial septal cholinergic neurons in mice. Neuroscience 2002 Abstracts 778.11. Society for Neuroscience, Orlando, FL.
Summary: We have shown previously that anti-murine-p75-SAP (saporin conjugated to a rat monoclonal antibody against the mouse p75 nerve growth factor receptor) selectively destroys basal forebrain cholinergic neurons in vivo after intracerebroventricular injections (J. Neurosci. 21:8164-73). Cholinergic neuronal loss was more extensive in the medial septum (MS) than the nucleus basalis magnocellularis; it is unclear whether this distinction is due to toxin diffusion from the ventricles or differential sensitivity of the neuronal populations. Intraparenchymal (IPC) injections to specific targets can help resolve the issue. Here, we examine the efficacy of anti-murine-p75-SAP IPC injections on cholinergic neurons. Saline or different doses of toxin (0.1, 0.2, 0.4, 0.9, 4.7, and 9.4 microg/microL) were injected into the MS of adult male C57BL/6J mice. Ten days post lesion, brain sections were stained for choline acetyltransferase and p75 (cholinergic markers) to determine toxin efficacy, and calbindin and parvalbumin (non-cholinergic markers) to determine toxin specificity. Toxin doses below 1.0 microg/microL had no effect on cholinergic or non-cholinergic neurons, while doses above 4.7 microg/microL resulted in the complete destruction of both cholinergic and non-cholinergic neurons. More thorough testing of doses between 1 and 4 microg/microL will be required to determine the optimal toxin dose for IPC injections.
Related Products: mu p75-SAP (Cat. #IT-16)