Ohara PT, Boudah A, Jasmin L (2002) Long-term effects on pain behavior of decreased spinal noradrenaline in neuropathic rats. Neuroscience 2002 Abstracts 351.22. Society for Neuroscience, Orlando, FL.
Summary: We sought to determine if a permanent reduction in the noradrenergic (NA) input to the spinal cord in adult rats would alter the pain behavior associated with nerve injury. Selective NA denervation of the lumbo-sacral cord was achieved by intrathecal injection of anti-dopamine beta-hydroxylase antibodies conjugated to the toxin saporin in 12 female rats. Spinal NA denervation was confirmed histologically in all animals. Saline injected rats served as controls. Two weeks after toxin or saline treatment, a unilateral peripheral neuropathy was induced by tight ligation of the left L5 spinal nerve in both groups. Unexpectedly, the same degree of mechanical hyperalgesia was present in the neuropathic paw of rats in both the toxin and saline treated groups. Rats lacking NA spinal afferents, however, were less responsive to the antinoiceptive effects of morphine administered systemically or intracerebroventricularly. Also, toxin treated rats did not display opioid dependant stress analgesia. Finally, toxin treated rats were more responsive to the antinociceptive effect of the NK1 antagonist CP 96,345 but not to its enantiomer CP 96,344. From these results we conclude that the permanent loss of spinal NA does not alter neuropathic pain behavior, possibly because of compensatory changes in the CNS. The decreased response to opioids is consistent with the previous suggestions of an interaction between noradrenergic and opioidergic systems in producing analgesia. The increased response to NK1 antagonists shows that NA tonically inhibits substance.
Related Products: Anti-DBH-SAP (Cat. #IT-03)