Nagle RA, Liberatore MA, Zombon NJ, Pokala VN, Li PK, Pokala VN, Johnson DA (2002) Effect of selective cholinergic lesioning of basal forebrain with 192 IgG-saporin on neurotransmitter concentrations in hippocampus of rat. Neuroscience 2002 Abstracts 880.6. Society for Neuroscience, Orlando, FL.
Summary: In vivo microdialysis techniques were used to examine the effects of lesioning of cholinergic neurons of the medial septum using the selective cholinergic neurotoxin 192-IgG-Saporin (SAP), on hippocampal acetylcholine (ACh), glutamate and GABA in adult male Sprague Dawley rats. High and low (1.0 and 0.22 μg) doses of SAP were used for infusion into the basal forebrain. SAP treated rats showed a significant dose dependent decrease of 74% and 59% in ACh for the high and low doses respectively, compared to controls. Glutamate decreased 50% in animals treated with 0.22 μg SAP. The data suggest that lesioning of basal forebrain neurons with SAP results in changes in neurotransmitter concentrations in the hippocampus.
Related Products: 192-IgG-SAP (Cat. #IT-01)