Pokala VN, Fitz NF, Witt-Enderby PA, Johnson DA (2004) Septohippocampal cholinergic lesion and hippocampal alpha-secretase activity in rat. Neuroscience 2004 Abstracts 846.14. Society for Neuroscience, San Diego, CA.
Summary: Previously we have shown that selective cholinergic lesion of the septohippocampal pathway in the rat resulted in a significant decrease in hippocampal extracellular acetylcholine (ACh) concentration, a compensatory increase in muscarinic receptor binding, but a decrease in muscarinic receptor-coupled G protein activation. The intent of this study was to investigate the effect of selective cholinergic lesion of the septohippocampal pathway on hippocampal alpha-secretase activity and expression. Alpha-secretase is an enzyme responsible for the proteolytic cleavage of amyloid protein precursor (APP) to release a neuroprotective soluble amyloid protein precursor (sAPP). Sprague-Dawley rats were infused into the medial septum with either the selective cholinergic immunotoxin 192 IgG-saporin (0.22 mg in 1ml aCSF) or vehicle. After 6 weeks the rats were euthanized and the hippocampus dissected from the brain and quickly frozen. Hippocampal homogenate was analyzed for alpha-secretase activity and expression. The results demonstrated an 80% decrease in alpha-secretase activity in SAP treated animals compared to control.
Related Products: 192-IgG-SAP (Cat. #IT-01)