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Effect of running on neurogenesis in cholinergic lesioned mice

Ho N, Han S, Dawe GS (2006) Effect of running on neurogenesis in cholinergic lesioned mice. Neuroscience 2006 Abstracts 318.5. Society for Neuroscience, Atlanta, GA.

Summary: Neurogenesis occurs mainly in two regions of the adult rodent brain, the dentate gyrus of the hippocampus and the subventricular zone. There are many factors which regulate neurogenesis, but voluntary exercise has consistently been shown to enhance neurogenesis. Exercise has been reported to specifically stimulate neural cell proliferation in the hippocampus but not the olfactory bulb. One of the major sources of afferents to the hippocampus are the septohippocampal projections, in which axons from the medial septum and diagonal band of Broca (MSDB) project to the hippocampus. Major components of the septohippocampal pathway that act as ‘pacemakers’ for hippocampal theta rhythm, which increases in conjunction with the voluntary running, are the cholinergic and GABAergic projections rising from cells in the MSDB. This present study investigates the effect of a partial cholinergic lesion in the basal forebrain and MSDB of mice, a partial model of the neurodegeneration that occurs in Alzheimer’s disease, on neural cell proliferation and neurogenesis. Murine p75-SAP, a conjugate of a p75 antibody that targets selectively cholinergic cells and cytotoxic saporin, was injected into the ventricles of female adult Swiss mice. After recovery from surgery mice were then administered bromodeoxyuridine (BrdU). BrdU immunopositive cells were quantified 24 hours and 4 weeks to assess for neural cell proliferation and survival of newly generated cells. Partial cholinergic denervation led to a decrease in the survival of new born cells in the dentate gyrus. We compared the effects of voluntary running for a period of 12 days in non-lesioned and lesioned mice under similar experimental conditions. Running resulted in an increase in neural cell proliferation for both the non-lesioned and lesioned groups. Running led to a marked increase in cell proliferation in lesioned mice compared to the controls, and also enhanced neurogenesis, as determined by the colocalization of BrdU and the neuronal nuclei marker NeuN in cells within the dentate gyrus. The present study suggests that voluntary running may have a positive effect on neurogenesis in neurodegenerative models in rodents. Further work needs to be done to elucidate the underlying mechanisms of exercise-induced neurogenesis.

Related Products: mu p75-SAP (Cat. #IT-16)

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