Abitoye PA, Li P, Gibbs RB, Johnson DA (2006) Steroid sulfatase inhibitor (p-O-sulfamoyl) – tetradecanoyl tyramine (du-14) enhances memory retention in rats with cholinergic lesion. Neuroscience 2006 Abstracts 163.15. Society for Neuroscience, Atlanta, GA.
Summary: Previous studies have shown that altering the metabolism of neurosteroids via inhibition of steroid sulfatase (SSI) would reverse scopolamine induced amnesia. In this study we tested whether the SSI, DU-14 could enhance memory retention of foot shock in rats with a selective lesion of cholinergic neurons projecting from the medial septum to the hippocampus using a passive avoidance paradigm. Male Sprague-Dawley rats were infused with either 0.2 μg of 192 IgG-saporin (SAP), a selective cholinergic immunotoxin, or artificial cerebrospinal fluid (CSF) into the medial septum. One week later, the animals were placed into a passive avoidance apparatus and administered footshock trials (1 mA / 1 sec) until criterion (2 consecutive trials with a crossover latency of at least 5 min). On the next day, rats from SAP and CSF groups were then randomly assigned to receive DU-14 (30mg / kg) or corn oil (vehicle) daily for 6 days. Rats were tested for memory retention three hours after the last day dosing. DU-14 increased crossover latency by 74.5% in the CSF control group and 54.8% in SAP treated animals. In order to determine whether DU-14 or SAP treatment inhibited locomotor activity independent of memory, other animals were dosed with vehicle or DU-14 and crossover latency was tested before acquisition of footshock. There were no significant differences between treatment groups. These results suggest that steroid sulfatase inhibition may enhance memory retention in rats with hippocampal cholinergic lesion.
Related Products: 192-IgG-SAP (Cat. #IT-01)