Cai L, Johnson DA (2007) Poster: Recognition of novel objects and their location in rats with selective cholinergic lesion of the medial septum. Neuroscience 2007 Abstracts 92.21/TT2. Society for Neuroscience, San Diego, CA.
Summary: The goal of this project was to determine whether cholinergic neurons projecting from the medial septum (MS) to the hippocampus play a role in novel object recognition or location. The specific aim was to determine whether lesion of cholinergic neurons in MS by the selective cholinergic neurotoxin 192IgG-saporin (SAP) would induce retrograde amnesia and/or anterograde amnesia for a novel object and/or its location. Male SD rats were tested in an object recognition paradigm. The time the rats spent examining old and novel objects was measured. Infusion of SAP into medial septum was performed 2 days after a one week pre-surgery training. Fourteen days after surgery post-surgery retention testing for retrograde object memory was carried out. Then 3 days later, a new acquisition training and retention testing for anterograde memory was started. One-way ANOVA and Fisher’s exact test were used for statistical analysis. There were no significant differences in the exploration ratios between the control group without surgery and the CSF surgical group. The mean exploration ratios for both groups demonstrated retention of memory for the novel object and its placement. SAP infusion into the MS failed to induce a deficit in retrograde amnesia 2 days after training, but did show a strong trend for anterograde amnesia for novel object recognition and a significant association with anterograde amnesia for object location. Conclusions were that cholinergic neurons of MS were not involved in retrograde object memory 2 days before the infusion of SAP and may or may not be necessary for anterograde object memory formation, but cholinergic neurons of the MS were involved in anterograde spatial memory formation for novel objects.
Related Products: 192-IgG-SAP (Cat. #IT-01)