Andäng M, Hjerling-Leffler J, Moliner A, Lundgren TK, Castelo-Branco G, Nanou E, Pozas E, Bryja V, Halliez S, Nishimaru H, Wilbertz J, Arenas E, Koltzenburg M, Charnay P, Manira AE, Ibañez CF, Ernfors P (2008) Histone H2AX-dependent GABAA receptor regulation of stem cell proliferation. Nature 451(7177):460-464. doi: 10.1038/nature06488 PMID: 18185516
Summary: Immunofluorescence staining for vesicular GABA transporter (VGAT) in embryonic stem cells reveals a punctate localization.
Usage: Immunostaining and western blotting. After being blocked for 2 h in PBS containing 3% BSA and 0.3% Triton X-100 and after two washes in PBS, cells were incubated in primary antibody against VGAT (diluted 1:400).
Related Products: vGAT Rabbit Polyclonal (Cat. #AB-N44)