Gertow K, Wolbank S, Rozell B, Sugars R, Andang M, Parish CL, Imreh MP, Wendel M, Ahrlund-Richter L (2004) Organized development from human embryonic stem cells after injection into immunodeficient mice. Stem Cells Dev 13:421-435. doi: 10.1089/scd.2004.13.421 PMID: 15345136
Summary: After thawing and brief drying, sections were fixed with 4% paraformaldehyde in PBS pH 7.4 for 40 min. Sections were blocked [PBS, 1% bovine serum albumin (BSA), 0.3% Triton X-100], washed, and incubated with primary antibodies for Tuj1 (-tubulin type III), glial fibrillary acid protein (GFAP), tyrosine hydroxylase (TH), and vesicular GABA transporter (vGAT; 1:400) overnight at 4°C. After rinsing in PBS, slides were incubated with Cy2- or Cy3-conjugated secondary antibodies.
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