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Targeted toxin-based selectable drug-free enrichment of Mammalian cells with high transgene expression.

Sato M, Akasaka E, Saitoh I, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S (2013) Targeted toxin-based selectable drug-free enrichment of Mammalian cells with high transgene expression. Biology (Basel) 2:341-355. doi: 10.3390/biology2010341

Summary: Cell transfection is a powerful tool for evaluation of function and expression of newly discovered genes as well as for both small and large scale eukaryotic expression of proteins. Most transfection strategies require a selection agent to eliminate cells that do not internalize the plasmid containing the gene of interest. Subsequent maintenance of the tranfected cells requires the presence of the selection agent, and the expression levels of the gene of interest have to be evaluated on a cell by cell basis. In this work the authors designed a system utilizing 50 μg/ml rIB4-SAP (Cat. #IT-10) to eliminate non-transfected cells and select for strong expression of the gene of interest. The data demonstrate that this technique will generate stable transfected cells that express the gene of interest at high levels.

Related Products: IB4-SAP (Cat. #IT-10)