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Lysophosphatidylcholine acyltransferase 1 protects against cytotoxicity induced by polyunsaturated fatty acids.

Akagi S, Kono N, Ariyama H, Shindou H, Shimizu T, Arai H (2016) Lysophosphatidylcholine acyltransferase 1 protects against cytotoxicity induced by polyunsaturated fatty acids. FASEB J 30:2027-2039. doi: 10.1096/fj.201500149

Summary: Dietary consumption of polyunsaturated fatty acids can influence the degree of fatty acid unsaturation in membrane phospholipids, and consequently membrane-associated functions. Scientists set out to investigate how mammalian cells change their membrane lipid composition in response to loading with excess polyunsaturated fatty acids (PUFAs). Lipidomic analysis showed that PUFA treatment induces production of dipalmitoylphosphatidylcholine (DPPC). By suppressing phospholipid metabolism-related genes by RNA interference, they found that Lysophosphatidylcholine acyltransferase 1 (LPCAT1) was involved in DPPC production. To reveal the role of DPPC produced by PUFA treatment, HeLa cells were transfected with a siRNA against LPCAT1 to reduce its protein expression. The cells were lysed after treatment with a PUFA and subjected to western blot analysis using a 1:1000 dilution of Anti-SCD-1 (Cat. #AB-259) as the primary. SCD-1 desaturates the substrate of LPCAT1 for producing DPPC. PUFAs significantly reduced both the protein and mRNA expression of SCD-1. They showed that inhibiting DPPC production by LPCAT1 knockdown enhanced apoptosis, suggesting that DPPC produced via LPCAT1 protects against PUFA-induced cytotoxicity.

Related Products: SCD-1 Mouse Monoclonal (Cat. #AB-259)

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