- ATS offers a custom biotinylation service to biotinylate antibodies and peptides, especially when customers plan to use them with our Streptavidin-ZAP.
- We have been seeing a trend of variability with commercially available antibodies, where customers will see over or under derivatization of material and this can be an issue if you plan to use it with Streptavidin-ZAP.
- There are a few fundamentals to look for when choosing/creating your biotinylated targeting agent:
- (1) the linker doesn’t impair target-binding affinity,
- (2) the linker should be stable and
- (3) the linker should be able to release payload efficiently.
- Important details to know about your biotinylated targeting agent:
- (1) the ratio of biotin to the protein,
- (2) unreacted biotin is purified away
- (3) the length of linker because long linkers could interfere with binding and
- (4) location of where biotin is attached such that important protein side-chains weren’t impacted.
- If any of these parameters are unfamiliar to you or you weren’t given these details, then I strongly encourage you to talk to us about a custom biotinylation service.
Note: discount code is expired