Works SJ, Wilson RE, Wellman CL (2004) Age-dependent effect of cholinergic lesion on dendritic morphology in rat frontal cortex. Neurobiol Aging 25(7):963-974. doi: 10.1016/j.neurobiolaging.2003.08.003 Summary: Aged rats display more dramatic and longer lasting effects due to brain injury than young animals. The authors examined the role cholinergic neurons may play in brain plasticity after injury […]
Gil-Bea FJ, Dominguez J, Garcia-Alloza M, Marcos B, Lasheras B, Ramirez MJ (2004) Facilitation of cholinergic transmission by combined treatment of ondansetron with flumazenil after cortical cholinergic deafferentation. Neuropharmacology 47(2):225-232. doi: 10.1016/j.neuropharm.2004.03.014 Summary: Previous studies from this group demonstrated that 5-HT(3) receptor antagonists potentiated by GABA(A) antagonists increased acetylcholine (ACh) release in the rat cerebral
It’s routine for primary neuronal cultures to have “contamination” with naturally occurring microglia. But what happens when you don’t want these little creatures or the cytokines and cytotoxins that they release? Or you want to eliminate any effect that microglia have on neurons or astrocytes? ATS has the cheap, effective and very simple solution for
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Advanced Targeting Systems announces its new fluorescent second antibody, FITC-labeled goat anti-rabbit IgG (Cat. #FL-04). This fluorescent conjugate is made with all the care that you expect from ATS. The figure above shows its use in flow cytometry on cells that express p75 and your results from immunohistochemistry should be just as clean. Look for
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Q: What dosage of 192-Saporin (192-IgG-SAP, Cat. #IT-01) should be used in the lateral ventricle to eliminate cholinergic neurons in the basal forebrain, including substantia innominata (SI)? I read that Calza et al [1] used 2 or 3 micrograms/4.5 µl and found this was highly effective. A: It has been our experience that two- or
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Stirpe F (2004) Featured Article: The discovery of saporin. Targeting Trends 5(3) Related Products: Saporin (Cat. #PR-01) Read the featured article in Targeting Trends.
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Chronic pain conditions are often caused by ongoing disease states or tissue damage that create sensitization of both the primary afferents (nerves that convey impulses from the outer part of the body) and spinal cord neurons. This sensitization results in a persistent increased sensitivity to both noxious (hyperalgesia) and non-noxious (allodynia) stimuli. Many persistent pain
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Contributed by Dr. Fiorenzo Stirpe, Dipartimento di Patologia sperimentale, Università di Bologna, I-40125 Bologna, Italy Saporin from Saponaria officinalis (soapwort plant of the Caryophyllaceae family) was discovered as part of research on plant toxins we undertook around 1970, when we became interested in the study of the mechanism of action of ricin. Ricin, from Ricinus
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Nishiguchi J, Sasaki K, Seki S, Chancellor MB, Erickson KA, de Groat WC, Kumon H, Yoshimura N (2004) Effects of isolectin B4-conjugated saporin, a targeting cytotoxin, on bladder overactivity induced by bladder irritation. Eur J Neurosci 20(2):474-482. doi: 10.1111/j.1460-9568.2004.03508.x Summary: It has been demonstrated that IB4-binding non-peptidergic C-fiber neuronal populations are present in afferent pathways
Hunter CL, Quintero EM, Gilstrap L, Bhat NR, Granholm AC (2004) Minocycline protects basal forebrain cholinergic neurons from mu p75-saporin immunotoxic lesioning. Eur J Neurosci 19(12):3305-3316. doi: 10.1111/j.0953-816X.2004.03439.x Summary: In Alzheimer’s disease basal cholinergic degeneration is accompanied by glial activation and the release of pro-inflammatory cytokines. To investigate whether neural events other than degeneration can