Targeting Stem Cells

Latest research using 192-IgG-SAP to eliminate p75-positive neurons.

Nitric Oxide Donor Molsidomine Promotes Retrieval of Object Recognition Memory in a Model of Cognitive Deficit Induced by 192 IgG-Saporin. (2019). Behav Brain Res, 366 108-117. Hernández-Melesio MA, Alcaraz-Zubeldia M, Jiménez-Capdeville ME, Martínez-Lazcano JC, Santoyo-Pérez ME, Quevedo-Corona L, Gerónimo-Olvera C, Sánchez-Mendoza A, Ríos C, & Pérez-Severiano F.  
Summary:  Results showed that 192-IgG-SAP reduced the immunoreactivity of cholinergic septal neurons (41%), compared with PBS-receiving control rats (p < 0.05).

Co-Treatment with Rivastigmine and Idalopirdine Reduces the Propensity for Falls in a Rat Model of Falls in Parkinson’s Disease. Lid – 10.1007/S00213-018-5150-Y [Doi]. (2019). Psychopharmacology (Berl), [Epub ahead of print] (1432-2072 (Electronic). Koshy Cherian A, Kucinski A, Wu R, de Jong IEM, & Sarter MA-Ohoo.
Objective: The authors used a longer and more taxing rotating beam apparatus to determine the potential therapeutic efficacy of idalopirdine when combined with  rivastigmine.
Summary: The results extend the prediction that the combined treatment with idalopirdine and an AChE inhibitor improves complex movement control and reduces propensity for falls in patients with movement disorders.
Dose: Basal forebrain cholinergic neurons situated in the nucleus basalis and substantia innominata were targeted with 192-IgG-SAP in aCSF infused bilaterally (200 ng/μL; 0.8 μL/hemisphere).

Basal Forebrain Chemogenetic Inhibition Disrupts the Superior Complex Movement Control of Goal-Tracking Rats. (2019). Behav Neurosci, 133 (1):121-134. 2019/01/29. Kucinski A, Kim Y, & Sarter M.
Dose: Basal forebrain cholinergic neurons situated in the nucleus basalis and substantia innominata were targeted with 192-IgG-SAP in aCSF infused bilaterally (200 ng/μL; 0.8 μL/hemisphere).

Partial Depletion of Septohippocampal Cholinergic Cells Reduces Seizure Susceptibility, but Does Not Mitigate Hippocampal Neurodegeneration in the Kainate Model of Epilepsy. (2019). Brain Res, 1717 235-246. Soares JI, Da Costa C, Ferreira MH, Andrade PA, Maia GH, & Lukoyanov NV.
Objective:  To examine how the inhibition of epilepsy-related cholinergic plasticity may be reflected inseizure susceptibility and/or in the development of chronic epilepsy and its neurological consequences.
Summary:  These data suggest that seizure-induced plasticity of cholinergic cells may indeed enhance seizure susceptibility and contribute to epileptogenic processes. They do not support the hypothesis that epilepsy-related hypertrophy of cholinergic neurons may potentiate hippocampal cell loss and respective behavioral impairments.
Dose:  Bilateral lesions of cholinergic cells were made by infusing 0.5 μl of SAP (0.08 μg/μl saline solution) into the hippocampus.

Disruption of Medial Septum and Diagonal Bands of Broca Cholinergic Projections to the Ventral Hippocampus Disrupt Auditory Fear Memory. (2018). Neurobiol Learning Memory, 152 71-79. Staib JM, Della Valle R, & Knox DK.
Objective:  To determine which efferent projections are critical for contextual fear memory discrimination and extinction memory.
Summary:  The results of this study suggest that MS/vDBB cholinergic neurons are critical for fear and extinction memory.
Dose:  192-IgG saporin was infused into all brain regions at a concentration of 0.2 μg/μL dissolved in 0.2 M  PBS. The total volume of each injection was 0.5 μL.  Sham surgeries were accomplished using the same volume (0.5 μL) of PBS.