ZAP Internalization Kit Tutorials

Please watch YouTube tutorials below for the ZAP Antibody Internalization Kit and Biotin-Z Kit.

ZAP Antibody Internalization Kit

  • The Kit  – Leonardo Ancheta, QC Scientist, provides an overview of what comes in the ZAP Antibody Internalization kits
  • Plate Preparation – Assay setup is covered, including how to add your cells to plates for the cytotoxicity assay according to the enclosed protocol
  • ZAP Plate – How to combine your antibody with the ZAP product for use in the assay.  Titration of your antibody and internal controls are also covered.
  • Control Plates – Every good assay has a positive and negative control.  In this video the use of Saporin and a Control toxin are covered.
  • Plate Development – The final step of the assay is described.  The mixing and addition of the developing reagents (XTT, PMS) as well as visual examples of developed plates.

Biotin-Z Kit

  • ZAP Intro – Brian Russell introduces the ZAP Internalization kits available from Advanced Targeting Systems
  • The Kit  – Leonardo Ancheta, QC Scientist, provides an overview of what comes in the ZAP Biotin-Z Internalization kit
  • Plate Preparation – Assay setup is covered, including how to add your cells to plates for the cytotoxicity assay according to the enclosed protocol
  • ZAP Plate – How to combine your biotinylated material with the Streptavidin-ZAP product for use in the assay.  Titration of your material and internal controls are also covered.
  • Control Plates – Every good assay has a positive and negative control.  In this video the use of Saporin alone as a control toxin is covered.
  • Plate Development – The final step of the assay is described.  The mixing and addition of the developing reagents (XTT, PMS) as well as visual examples of developed plates.