Please watch YouTube tutorials below for the ZAP Antibody Internalization Kit and Biotin-Z Kit.
ZAP Antibody Internalization Kit
2 AB ZAP Kit
ZAP Plate Preparation
4 - Ab ZAP Plate
5 - Preparing Controls-Ab
6 - Kit Develop - Ab
- ZAP Intro – Brian Russell introduces the ZAP Internalization kits available from Advanced Targeting Systems
- The Kit – Leonardo Ancheta, QC Scientist, provides an overview of what comes in the ZAP Antibody Internalization kits
- Plate Preparation – Assay setup is covered, including how to add your cells to plates for the cytotoxicity assay according to the enclosed protocol
- ZAP Plate – How to combine your antibody with the ZAP product for use in the assay. Titration of your antibody and internal controls are also covered.
- Control Plates – Every good assay has a positive and negative control. In this video the use of Saporin and a Control toxin are covered.
- Plate Development – The final step of the assay is described. The mixing and addition of the developing reagents (XTT, PMS) as well as visual examples of developed plates.
Biotin-Z Kit
1 - KIT-Z Biotin Intro
2 - Biotin-Z - The Kit
3 - Plate Preparation-Biotin-Z
4 - Preparing Strep-ZAP Plate
5 - Preparing Controls - Biotin-Z
6 - Kit Develop - Biotin
- ZAP Intro – Brian Russell introduces the ZAP Internalization kits available from Advanced Targeting Systems
- The Kit – Leonardo Ancheta, QC Scientist, provides an overview of what comes in the ZAP Biotin-Z Internalization kit
- Plate Preparation – Assay setup is covered, including how to add your cells to plates for the cytotoxicity assay according to the enclosed protocol
- ZAP Plate – How to combine your biotinylated material with the Streptavidin-ZAP product for use in the assay. Titration of your material and internal controls are also covered.
- Control Plates – Every good assay has a positive and negative control. In this video the use of Saporin alone as a control toxin is covered.
- Plate Development – The final step of the assay is described. The mixing and addition of the developing reagents (XTT, PMS) as well as visual examples of developed plates.