ZAP Internalization Kit Tutorials

Please watch YouTube tutorials below for the ZAP Antibody Internalization Kit and Biotin-Z Kit.

ZAP Antibody Internalization Kit

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A description of the components in an Antibody Internalization Kit by Advanced Targeting Systems. This kit is used to screen antibodies for their specificity and ability to internalize. The technology uses a secondary antibody conjugated to saporin. Simply mix the Secondary ZAP conjugate with your primary antibody to make your own targeted toxin. Apply the targeted toxin to cells that express the receptor your antibody is targeting. Develop the cytotoxicity assay to determine whether your antibody recognizes the target receptor. This assay is used to screen multiple antibodies for the same target to determine which is the best antibody to continue development as a drug or research tool.

  • AB ZAP Kit 1 – The Kit (overview of what comes in the kit)
  • AB ZAP Kit 2 – Plate Preparation (preparing plates for cytotoxicity assay)
  • AB ZAP Kit 3 – Adding ZAP (adding ZAP conjugate to the plates)
  • AB ZAP Kit 4 – Control Plates (adding control conjugate to the plates)
  • AB ZAP Kit 5 – Plate Development (developing the cytotoxicity assay)

Biotin-Z Kit

  • The Kit  – Leonardo Ancheta, QC Scientist, provides an overview of what comes in the ZAP Biotin-Z Internalization kit
  • Plate Preparation – Assay setup is covered, including how to add your cells to plates for the cytotoxicity assay according to the enclosed protocol
  • ZAP Plate – How to combine your biotinylated material with the Streptavidin-ZAP product for use in the assay. Titration of your material and internal controls are also covered.
  • Control Plates – Every good assay has a positive and negative control. In this video the use of Saporin alone as a control toxin is covered.
  • Plate Development – The final step of the assay is described. The mixing and addition of the developing reagents (XTT, PMS) as well as visual examples of developed plates.