Technology Tutorial

Advanced Targeting Systems’ technology – Molecular Surgery – is a modification of one of the most widely used techniques in the neurosciences: lesioning of a region by surgical means and observation of the effect.

Saporin has no way of entering a cell on its own, except by bulk-phase endocytosis. To be effective, Saporin is bound to a Targeting Agent. A Targeting Agent binds to the cell surface and is internalized. Examples include: antibody, ligand, cytokine, growth factor, aptamer, etc. When the conjugate is internalized it escapes the endosomal compartment and inactivates the ribosomes. This shuts down protein synthesis and results in cell death.

1. Binding to Cell Surface Marker; 2. Internalization; 3. Escape from Endosome; 4. Inactivation of Ribosomes; The result is cell death.