pHast Conjugates
pHast Conjugates are one of our pHastest tools for quantitative testing of your primary antibody’s specificity, binding, and internalization, providing results in 1 day.
How It Works
The pHast conjugate binds to your primary antibody via a secondary antibody cross-linked to a pH-dependent fluorescent reporter. This fluorescent reporter will increase intensity as the pH of its surroundings becomes more acidic, as evident when exposed to the environment inside a cell. pHast conjugates are fluorescent plate reader compatible and can be used to illuminate your lead antibody candidates in a 96-well plate format.
Also Available:
Streptavidin-pHast (PH-03) 
Streptavidin-pHast binds to your biotinylated protein and contains a pH-dependent fluorescent reporter (pHast).
All of our pHast Conjugates are available with these fluorescent dyes.
- The pHast RED has an excitation wavelength of 532 nm with an emission maxima at 560 nm.
- The pHast FAR-RED has an excitation wavelength of 643 nm with an emission maxima at 660 nm.
- The pHast GREEN version has an excitation wavelength of 453 nm with an emission maxima at 522 nm.
- The pHast BLUE has an excitation wavelength of 362 nm with an emission maxima at 452 nm.
pHast Multi Color Kit
Get all four of our pHast conjugates colors in one bundle – 100 µg vials of each.
Our new kit provides a matched set of Blue, Green, Red, and Far Red pHast conjugates.
Choose your target below to order individual colors or the multi color kit.
| Target | Color | Recommended Products made with whole IgG | made with monovalent (Fab) | made with monovalent Fc region (FabFc) |
|---|---|---|---|---|
| alpaca/llama IgG (VHH domain) | | Alpaca-pHast (VHH) (PH-05) | Fab-pHast Alpaca (VHH) (PH-21) | |
| alpaca/llama IgG | | Alpaca-pHast (IgG) (PH-20) | ||
| chicken IgY | | Chick-pHast (PH-06) | ||
| goat IgG | | Goat-pHast (PH-07) | ||
| guinea pig IgG | | gPIG-pHast (PH-08) | ||
| human IgG | | Hum-pHast (PH-09) | Fab-pHast human (PH-01) | FabFc-pHast human (PH-10) |
| human IgM | | Hug-M-pHast (PH-11) | FabFc-pHast Hug-M (PH-04) | |
| mouse IgG | | Mab-pHast (PH-12) | Fab-pHast mouse (PH-02) | FabFc-pHast mouse (PH-13) |
| mouse IgM | | Anti-M-pHast (PH-14) | ||
| rabbit IgG | | Rab-pHast (PH-15) | Fab-pHast rabbit (PH-16) | |
| rat IgG | | Rat-pHast (PH-17) | Fab-pHast rat (PH-18) | FabFc-pHast rat (PH-19) |
 made with bivalent antibodies that recognize the whole IgG |  made with monovalent (Fab) antibodies that recognize the whole IgG, without bivalent capping |  made with monovalent (Fab) antibodies that recognize Fc region only |
View a complete product listing here.