The use of polyhistidine tags has become a popular method for protein purification, commonly used in the screening process as a tag for your protein or peptide of interest. This polyhistidine epitope tag is generally comprised of six consecutive histidine amino acid residues located at the N-terminal, C-terminal, or internally. The 6-His-Tag is widely used because of its affinity to bind nickel or cobalt metal ions attached to sepharose, which can then be used to purify the protein in a native or denatured state.
Anti-6-His-SAP is a chemical conjugate of the mouse monoclonal antibody to 6 His and the ribosome-inactivating protein, saporin. It specifically eliminates cells with extracellular expression of 6 His-tagged proteins and cells that recognize YOUR 6 His-tagged recombinant proteins.
Anti-6-His-SAP is available individually (Cat. #IT-52) or as a kit (Cat. #KIT-52) which includes Anti-6-His-SAP and Mouse IgG-SAP (Cat. #IT-18).
keywords: 6-His-Tag, 6-His, 6His, N-terminal, C-terminal, His-tagged, nickel, cobalt, Anti-6His, Anti-6-His, saporin, secondary
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