Anti-6 His-SAP [IT-52, KIT-52]

a tool for eliminating 6 His-expressing cells; targeted via mouse monoclonal antibody to 6 His, eliminated via saporin

SKU: IT-52 Category: Antibody Type: Monoclonal | Host: mouse |Conjugate: saporin | Usage: eliminates cells |

The use of polyhistidine tags has become a popular method for protein purification, commonly used in the screening process as a tag for your protein or peptide of interest. This polyhistidine epitope tag is generally comprised of six consecutive histidine amino acid residues located at the N-terminal, C-terminal, or internally. The 6-His-Tag is widely used because of its affinity to bind nickel or cobalt metal ions attached to sepharose, which can then be used to purify the protein in a native or denatured state.

Anti-6-His-SAP is a chemical conjugate of the mouse monoclonal antibody to 6 His and the ribosome-inactivating protein, saporin. It specifically eliminates cells with extracellular expression of 6 His-tagged proteins and cells that recognize YOUR 6 His-tagged recombinant proteins.

Anti-6-His-SAP is available individually (Cat. #IT-52) or as a kit (Cat. #KIT-52) which includes Anti-6-His-SAP and Mouse IgG-SAP (Cat. #IT-18).