Blank-Strep-SAP is the perfect control for use with peptide conjugates utilizing biotin linkage to Streptavidin-ZAP (IT-27). It consists of a non-targeted peptide that has been biotinylated and mixed with Streptavidin-ZAP. The sequence of the non-targeted peptide of this molecule is an 11-amino acid, randomly mixed version of the sequence of melanocyte-stimulating hormone, with amino acid residues that are typical of peptides that bind to G-protein-coupled receptors. Examination of the peptide sequence using NCBI/BLAST reveals no homologous sequences. Thus, Blank-Strep-SAP can be used as control for any non-specific effects of the toxin and provides a definitive baseline for determining the effects of a targeted peptide conjugate.
Blank-Strep-SAP serves as a control for peptide-targeted toxins utilizing biotin-streptavidin linkage (Nppb-SAP).