The dopamine transporter (DAT) is a sodium-dependent reuptake carrier that is suspected to play a role in such neurologic and psychiatric disorders as Parkinson’s disease, Tourette’s syndrome, schizophrenia, and addiction. It is a 12-transmembrane domain transporter with the N- and C-terminus regions located within the cytoplasm. Because of its importance in dopamine use, DAT is an identifier of dopaminergic neurons. Specific elimination of cells that express DAT is useful in studying the role of dopaminergic neurons in one of the devastating diseases of old age, Parkinson’s.
Anti-DAT-SAP is a chemical conjugate of a rat monoclonal antibody to the second extracellular loop of the dopamine transporter (DAT-ECD) and the ribosome-inactivating protein, saporin. It is highly specific for cells that express DAT, including mesencephalic dopamine neurons of the substantia nigra, pars compacta, and ventral tegmental area.