Dopamine Transporter Rat Monoclonal (DAT-ECD), FITC-labeled [AB-N17-FL]

FITC-labeled Anti-DAT-ECD can be used to verify binding specificity of a targeted toxin to a cell line expressing the target molecule. By first binding the targeted toxin to fixed cells, then binding FITC-labeled Anti-DAT-ECD to the targeted toxin, specificity can be confirmed through the use of competing molecules or a control cell line. FITC is excited by 488 nm wavelength light, and emits at 525 nm.

This antibody recognizes the second extracellular loop of the dopamine transporter (DAT-ECD) in rat and human. It was produced in rat by immunization with a GST-DAT-loop fusion protein, then construction of a hybridoma with the murine nonsecreting myeloma cell line Sp2/0. The second extracellular loop, consisting of amino acids 180-218, was used to construct the fusion protein. The antibody was conjugated to FITC (5-iodoacetamidofluorescein) using SPDP.

Applications include flow cytometry.

Dopamine Transporter Products:

Dopamine Transporter Rat Monoclonal (DAT-ECD) (Cat. #AB-N17) | Alexa488-labeled (Cat. #AB-N17-FLA) | biotin-labeled (Cat. #AB-N17-BT) | FITC-labeled (Cat. #AB-N17-FL)

Dopamine Transporter Rat Monoclonal (DAT-NT) (Cat. #AB-N18) | Alexa488-labeled (Cat. #AB-N18-FLA) | biotin-labeled (Cat. #AB-N18-BT)

Anti-DAT-SAP (Cat. #IT-25)

keywords: dopamine transporter, dat, dat-ecd, extracellular domain, Anti-DAT-ECD, Anti-Dopamine Transporter, fluorescent, FITC, brain, neuroscience