Dopamine Transporter Rat Monoclonal (DAT-ECD), biotin labeled [AB-N17-BT]

The dopamine transporter is a sodium-dependent reuptake carrier that is suspected to play a role in such neurologic and psychiatric disorders as Parkinson’s disease, Tourette’s disease, schizophrenia, and addiction. It is a12-transmembrane domain transporter with the N- and C- terminus regions located within the cytoplasm. This antibody to the dopamine transporter was originally designated DAT/e2 and is now termed anti-DAT-ECD, emphasizing that it recognizes an extracellular domain.

This antibody recognizes the second extracellular loop of the dopamine transporter (DAT-ECD) in rat and human. It was produced in rat by immunization with a GST-DAT-loop fusion protein, then construction of a hybridoma with the murine nonsecreting myeloma cell line Sp2/0. The second extracellular loop, consisting of amino acids 180-218, was used to construct the fusion protein. It has been conjugated to biotin via an amide bond.

Applications include immunocytochemistry, immunohistochemistry, and immunoblotting.

Dopamine Transporter Products:

Dopamine Transporter Rat Monoclonal (DAT-ECD) (Cat. #AB-N17) | Alexa488-labeled (Cat. #AB-N17-FLA) | biotin-labeled (Cat. #AB-N17-BT) | FITC-labeled (Cat. #AB-N17-FL)

Dopamine Transporter Rat Monoclonal (DAT-NT) (Cat. #AB-N18) | Alexa488-labeled (Cat. #AB-N18-FLA) | biotin-labeled (Cat. #AB-N18-BT)

Anti-DAT-SAP (Cat. #IT-25)

keywords: dopamine transporter, dat, dat-ecd, extracellular domain, Anti-DAT-ECD, Anti-Dopamine Transporter, biotin, brain, neuroscience