Dopamine Transporter Rat Monoclonal (DAT-ECD) [AB-N17]

Name: Dopamine Transporter Rat Monoclonal (DAT-ECD) [AB-N17]
SKU: AB-N17
Availability: InStock

$394.00

The dopamine transporter is a sodium-dependent reuptake carrier that is suspected to play a role in such neurologic and psychiatric disorders as Parkinson’s disease, Tourette’s disease, schizophrenia, and addiction. It is a12-transmembrane domain transporter with the N- and C- terminus regions located within the cytoplasm. This antibody to the dopamine transporter was originally designated DAT/e2 and is now termed anti-DAT-ECD, emphasizing that it recognizes an extracellular domain.

This antibody recognizes the second extracellular loop of the dopamine transporter (DAT-ECD) in rat and human. It was produced in rat by immunization with a GST-DAT-loop fusion protein, then construction of a hybridoma with the murine nonsecreting myeloma cell line Sp2/0. The second extracellular loop, consisting of amino acids 180-218, was used to construct the fusion protein.

Applications include immunocytochemistry, immunohistochemistry, immunoblotting, and targeting.

keywords: dopamine transporter, dat, dat-ecd, extracellular domain, Anti-DAT-ECD, Anti-Dopamine Transporter, Parkinson’s disease, PD, brain, neuroscience

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View Data Sheet

Estradiol effects on the dopamine transporter – protein levels, subcellular location, and function.

Watson CS, Alyea RA, Hawkins BE, Thomas ML, Cunningham KA, Jakubas AA (2006) Estradiol effects on the dopamine transporter - protein levels, subcellular location, and function. J Mol Signal 1:5. doi: 10.1186/1750-2187-1-5 PMID: 17224081

Related Products: Dopamine Transporter Rat Monoclonal (DAT-ECD) (Cat. #AB-N17)

Dopamine transporter-immunoreactive neurons decrease with age in the human substantia nigra.

Ma SY, Ciliax BJ, Stebbins G, Jaffar S, Joyce JN, Cochran EJ, Kordower JH, Mash DC, Levey AI, Mufson EJ (1999) Dopamine transporter-immunoreactive neurons decrease with age in the human substantia nigra. J Comp Neurol 409(1):25-37. doi: 10.1002/(sici)1096-9861(19990621)409:13.0.co;2-e PMID: 10363709

Objective: To quantify the number of DAT immunostained neurons.

Summary: Findings demonstrate an age-related reduction in the number of substantia nigra DAT-immunoreactive neurons.

Usage: IHC (1:500)

Related Products: Dopamine Transporter Rat Monoclonal (DAT-ECD) (Cat. #AB-N17), Dopamine Transporter Rat Monoclonal (DAT-NT) (Cat. #AB-N18)

Subcellular localization and molecular topology of the dopamine transporter in the striatum and substantia nigra.

Hersch SM, Yi H, Heilman CJ, Edwards RH, Levey AI (1997) Subcellular localization and molecular topology of the dopamine transporter in the striatum and substantia nigra. J Comp Neurol 388(2):211-227. PMID: 9368838

Usage: Western Blot (1:500) and IHC (1:100–1:250)

Related Products: Dopamine Transporter Rat Monoclonal (DAT-ECD) (Cat. #AB-N17), Dopamine Transporter Rat Monoclonal (DAT-NT) (Cat. #AB-N18)

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Dopamine Transporter Products:

Dopamine Transporter Rat Monoclonal (DAT-ECD) (Cat. #AB-N17) | Alexa488-labeled (Cat. #AB-N17-FLA) | biotin-labeled (Cat. #AB-N17-BT) | FITC-labeled (Cat. #AB-N17-FL)

Dopamine Transporter Rat Monoclonal (DAT-NT) (Cat. #AB-N18) | Alexa488-labeled (Cat. #AB-N18-FLA) | biotin-labeled (Cat. #AB-N18-BT)